6VHQ
Crystal structure of Bacillus subtilis levansucrase (D86A/E342A) in complex with oligosaccharides
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Glycoside hydrolase family 68 protein | polymer | 466 | 52360.8 | 2 | UniProt (A0PFL2) Pfam (PF02435) UniProt (by SIFTS) (P05655) In PDB | Bacillus subtilis | Levansucrase |
2 | C, D, E | beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose | branched | 990.9 | 3 | In PDB GlyTouCan (G00825VL) | |||
3 | F | beta-D-fructofuranose-(2-6)-beta-D-fructofuranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900051) GlyTouCan (G51829YA) | levanbiose | ||
4 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
5 | A, B | BROMIDE ION | non-polymer | 79.9 | 9 | Chemie (BR) | |||
6 | water | water | 18.0 | 540 | Chemie (HOH) |
Sequence modifications
A, B: 30 - 473 (UniProt: A0PFL2)
PDB | External Database | Details |
---|---|---|
Ala 86 | Asp 86 | engineered mutation |
Ala 342 | Glu 342 | engineered mutation |
Thr 474 | - | expression tag |
Asp 475 | - | expression tag |
Pro 476 | - | expression tag |
Asn 477 | - | expression tag |
Ser 478 | - | expression tag |
Ser 479 | - | expression tag |
Ser 480 | - | expression tag |
Val 481 | - | expression tag |
Asp 482 | - | expression tag |
Lys 483 | - | expression tag |
Leu 484 | - | expression tag |
Ala 485 | - | expression tag |
Ala 486 | - | expression tag |
Ala 487 | - | expression tag |
Leu 488 | - | expression tag |
Glu 489 | - | expression tag |
His 490 | - | expression tag |
His 491 | - | expression tag |
His 492 | - | expression tag |
His 493 | - | expression tag |
His 494 | - | expression tag |
His 495 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 104721.6 | |
Branched | Number of molecules | 4 |
Total formula weight | 3314.9 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 799.3 | |
All* | Total formula weight | 108835.8 |