6T79

Structure of a human nucleosome at 3.2 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.2	polymer	136	15389.0	2	UniProt (Q71DI3) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H3/m,Histone H3/o
2	B, F	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	C, G	Histone H2A type 1-B/E	polymer	151	16707.3	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H	Histone H2B type 1-K	polymer	126	13921.2	2	UniProt (O60814) Pfam (PF00125) In PDB	Homo sapiens (Human)	H2B K,HIRA-interacting protein 1
5	I	DNA (147-MER)	polymer	147	45240.8	1		synthetic construct
6	J	DNA (147-MER)	polymer	147	45484.3	1		synthetic construct

Sequence modifications

A, E: 0 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

C, G: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Ser -5	-	expression tag
Asn -4	-	expression tag
Ala -3	-	expression tag
Pro -2	-	expression tag
Trp -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	205549.0
All*	Total formula weight	205549.0

*Water molecules are not included.