6T74

New antiparallel dimer of aureochrome 1a LOV domain mutants from Phaeodactylum tricornutum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ptaureo1a lov2 domain	polymer	162	18078.9	2	UniProt (A0A140UHJ0) Pfam (PF13426) In PDB	Phaeodactylum tricornutum (Diatom)
2	A, B	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	A, B	PENTAETHYLENE GLYCOL	non-polymer		238.3	3	Chemie (1PE)
4	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	B	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
7		water	water		18.0	323	Chemie (HOH)

Sequence modifications

A, B: 237 - 378 (UniProt: A0A140UHJ0)

PDB	External Database	Details
Met 217	-	initiating methionine
Gly 218	-	expression tag
Ser 219	-	expression tag
Ser 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
Ser 227	-	expression tag
Ser 228	-	expression tag
Gly 229	-	expression tag
Leu 230	-	expression tag
Val 231	-	expression tag
Pro 232	-	expression tag
Arg 233	-	expression tag
Gly 234	-	expression tag
Ser 235	-	expression tag
His 236	-	expression tag
Trp 349	Val 113	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	36157.8
Non-Polymers*	Number of molecules	10
	Total formula weight	1959.7
All*	Total formula weight	38117.5

*Water molecules are not included.