6RIN

Cryo-EM structure of E. coli RNA polymerase backtracked elongation complex bound to GreB transcription factor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	N	Non-template DNA	polymer	39	11962.7	1		Escherichia coli
2	T	Template DNA	polymer	39	12065.7	1		Escherichia coli
3	F	Transcription elongation factor GreB	polymer	158	18573.1	1	UniProt (C3SQ22) Pfam (PF03449) Pfam (PF01272) UniProt (by SIFTS) (P30128) In PDB	Escherichia coli K12	Transcript cleavage factor GreB
4	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
5	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
6	D	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
7	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8	R	RNA	polymer	14	4441.6	1		Escherichia coli
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	436597.7
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	436752.9

*Water molecules are not included.