6R25

Structure of LSD2/NPAC-linker/nucleosome core particle complex: Class 3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	K	Lysine-specific histone demethylase 1B	polymer	776	87051.5	1	UniProt (Q8NB78) Pfam (PF07496) Pfam (PF04433) Pfam (PF01593) In PDB	Homo sapiens (Human)	Flavin-containing amine oxidase domain-containing protein 1,Lysine-specific histone demethylase 2
2	L	NPAC	polymer	12	1481.6	1	UniProt (by SIFTS) (Q49A26) In PDB	Homo sapiens
3	M	Histone H3	polymer	135	15305.9	1	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) In PDB	Xenopus laevis (African clawed frog)
4	A, E	Histone H3	polymer	135	15303.9	2	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) In PDB	Xenopus laevis (African clawed frog)
5	B, F	H4	polymer	102	11263.2	2	Pfam (PF15511) UniProt (by SIFTS) (P62799) In PDB	Xenopus laevis (African clawed frog)
6	C, G	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) In PDB	Xenopus laevis (African clawed frog)
7	D, H	H2B	polymer	126	13939.2	2	Pfam (PF00125) UniProt (by SIFTS) (P02281) In PDB	Xenopus laevis (African clawed frog)
8	I	DNA (147-MER)	polymer	147	45610.0	1		synthetic construct
9	J	DNA (147-MER)	polymer	147	45138.8	1		synthetic construct
10	K	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
11	K	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
12		water	water		18.0	314	Chemie (HOH)

Sequence modifications

K: 51 - 822 (UniProt: Q8NB78)

PDB	External Database	Details
Pro 47	-	expression tag
Leu 48	-	expression tag
Gly 49	-	expression tag
Ser 50	-	expression tag

M: 1 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Met 4	Lys 5	conflict

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	303557.2
Non-Polymers*	Number of molecules	4
	Total formula weight	981.8
All*	Total formula weight	304539.0

*Water molecules are not included.