6QTB

Crystal structure of Rea1-MIDAS/Ytm1-UBL complex from Chaetomium thermophilum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Midasin,Midasin	polymer	280	31464.8	2	UniProt (G0SHE6) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
2	B, E	Ribosome biogenesis protein YTM1	polymer	101	11444.0	2	UniProt (G0SFB5) Pfam (PF08154) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
3	A, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, D, E	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5		water	water		18.0	406	Chemie (HOH)

Sequence modifications

A, D: 4690 - 4733 (UniProt: G0SHE6)

PDB	External Database	Details
Met 4689	-	initiating methionine

A, D: 4774 - 4997 (UniProt: G0SHE6)

PDB	External Database	Details
Gly 4734	-	linker
Ser 4735	-	linker
Gly 4773	-	linker
Gly 4998	-	expression tag
Ser 4999	-	expression tag
His 5000	-	expression tag
His 5001	-	expression tag
His 5002	-	expression tag
His 5003	-	expression tag
His 5004	-	expression tag
His 5005	-	expression tag

B, E: 8 - 98 (UniProt: G0SFB5)

PDB	External Database	Details
Met -2	-	initiating methionine
Lys -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Pro 6	-	expression tag
Met 7	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	85817.7
Non-Polymers*	Number of molecules	5
	Total formula weight	324.9
All*	Total formula weight	86142.6

*Water molecules are not included.