6QNO
Rhodopsin-Gi protein complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 376 | 43182.1 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62871) Pfam (PF00400) In PDB | Bos taurus (Bovine) | Transducin beta chain 1 |
| 3 | G | Guanine nucleotide-binding protein G(T) subunit gamma-T1 | polymer | 74 | 8556.9 | 1 | UniProt (P02698) Pfam (PF00631) In PDB | Bos taurus (Bovine) | Transducin gamma chain |
| 4 | L | Fab antibody fragment light chain | polymer | 239 | 26309.6 | 1 | Mus musculus (House Mouse) | ||
| 5 | H | Fab antibody fragment heavy chain | polymer | 249 | 26558.9 | 1 | Mus musculus (House Mouse) | ||
| 6 | R | Rhodopsin | polymer | 348 | 39040.5 | 1 | UniProt (P02699) Pfam (PF10413) Pfam (PF00001) In PDB | Bos taurus (Bovine) | |
| 7 | C | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 8 | R | RETINAL | non-polymer | 284.4 | 1 | Chemie (RET) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
R: 1 - 348 (UniProt: P02699)
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | initiating methionine |
| Lys -20 | - | expression tag |
| Lys -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys 2 | Asn 2 | engineered mutation |
| Tyr 257 | Met 257 | engineered mutation |
| Cys 282 | Asp 282 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 181064.9 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 424.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 284.4 | |
| All* | Total formula weight | 181773.7 |
