6PWQ
Crystal structure of Levansucrase from Bacillus subtilis mutant S164A at 2.6 A
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Glycoside hydrolase family 68 protein | polymer | 466 | 52446.8 | 2 | UniProt (A0PFL2) Pfam (PF02435) UniProt (by SIFTS) (P05655) In PDB | Bacillus subtilis | Levansucrase |
2 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
3 | B, A | SULFATE ION | non-polymer | 96.1 | 20 | Chemie (SO4) | |||
4 | B, A | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
5 | B, A | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
6 | water | water | 18.0 | 187 | Chemie (HOH) |
Sequence modifications
A, B: 30 - 473 (UniProt: A0PFL2)
PDB | External Database | Details |
---|---|---|
Ala 164 | Ser 164 | engineered mutation |
Thr 474 | - | expression tag |
Asp 475 | - | expression tag |
Pro 476 | - | expression tag |
Asn 477 | - | expression tag |
Ser 478 | - | expression tag |
Ser 479 | - | expression tag |
Ser 480 | - | expression tag |
Val 481 | - | expression tag |
Asp 482 | - | expression tag |
Lys 483 | - | expression tag |
Leu 484 | - | expression tag |
Ala 485 | - | expression tag |
Ala 486 | - | expression tag |
Ala 487 | - | expression tag |
Leu 488 | - | expression tag |
Glu 489 | - | expression tag |
His 490 | - | expression tag |
His 491 | - | expression tag |
His 492 | - | expression tag |
His 493 | - | expression tag |
His 494 | - | expression tag |
His 495 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 104893.7 | |
Non-Polymers* | Number of molecules | 29 |
Total formula weight | 2850.3 | |
All* | Total formula weight | 107744.0 |