6PED

Crystal structure of HEMK2-TRMT112 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Methyltransferase N6AMT1	polymer	233	25011.6	1	UniProt (Q9Y5N5) Pfam (PF05175) In PDB	Homo sapiens (Human)	HemK methyltransferase family member 2,M.HsaHemK2P,Methylarsonite methyltransferase N6AMT1,N(6)-adenine-specific DNA methyltransferase 1,Protein N(5)-glutamine methyltransferase
2	B	Multifunctional methyltransferase subunit TRM112-like protein	polymer	143	16415.7	1	UniProt (Q9UI30) Pfam (PF03966) In PDB	Homo sapiens (Human)	tRNA methyltransferase 112 homolog
3	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
4	A, B	UNKNOWN ATOM OR ION	non-polymer			12	Chemie (UNX)

Sequence modifications

A: 1 - 214 (UniProt: Q9Y5N5)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 1 - 125 (UniProt: Q9UI30)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Arg -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	41427.3
Non-Polymers*	Number of molecules	13
	Total formula weight	384.4
All*	Total formula weight	41811.8

*Water molecules are not included.