6PA9
E. coli L-asparaginase II mutant (T12V) in complex with L-Asn at pH 7.0
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | L-asparaginase 2 | polymer | 334 | 35700.0 | 2 | UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB | Escherichia coli (strain K12) | L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II |
2 | A, B | ASPARAGINE | non-polymer | 132.1 | 2 | Chemie (ASN) | |||
3 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
4 | water | water | 18.0 | 486 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 326 (UniProt: P00805)
PDB | External Database | Details |
---|---|---|
Met -7 | - | initiating methionine |
Asp -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
Val 12 | Thr 34 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 71400.0 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 356.3 | |
All* | Total formula weight | 71756.3 |