6P7E

Structure of T7 DNA Polymerase Bound to a Primer/Template DNA and a Peptide that Mimics the C-terminal Tail of the Primase-Helicase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	DNA-directed DNA polymerase	polymer	704	79703.6	4	UniProt (P00581) Pfam (PF00476) In PDB	Enterobacteria phage T7 (Bacteriophage T7)	Gene product 5,Gp5
2	E, F, G, H	TrxA	polymer	109	11818.6	4	UniProt (Q14F07) Pfam (PF00085) UniProt (by SIFTS) (P0AA25) In PDB	Escherichia coli	Thioredoxin 1
3	I, K, M, O	DNA (5'-D(P*GP*GP*CP*AP*GP*GP*TP*GP*GP*TP*CP*TP*TP*GP*CP*CP*GP*GP*TP*GP*A)-3')	polymer	21	6551.2	4		Enterobacteria phage T7
4	J, L, N, P	DNA (25-MER)	polymer	25	7518.9	4		Enterobacteria phage T7
5	U, V	ASP-THR-ASP-PHE peptide	polymer	4	496.5	2		Enterobacteria phage T7
6	W	THR-ASP-PHE peptide	polymer	3	381.4	1		Enterobacteria phage T7
7	B, C, D, A	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	8	Chemie (TTP)
8	B, C, D, A	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)
9		water	water		18.0	31	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 704 (UniProt: P00581)

PDB	External Database	Details
Ala 5	Asp 5	engineered mutation
Ala 7	Glu 7	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	19
	Total formula weight	423743.2
Non-Polymers*	Number of molecules	20
	Total formula weight	4149.0
All*	Total formula weight	427892.2

*Water molecules are not included.