6O6X

Crystal structure of Csm6 W14A/E337A mutant in complex with cA4 by cocrystallization

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Csm6	polymer	440	49621.9	2	UniProt (B6YWC3) Pfam (PF22230) In PDB	Thermococcus onnurineus (strain NA1)
2	C, D	Cyclic RNA cA4	polymer	4	1271.9	2		synthetic construct	RNA (5'-R(P*AP*AP*AP*A)-3')
3		water	water		18.0	96	Chemie (HOH)

Sequence modifications

A, B: 1 - 432 (UniProt: B6YWC3)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ala 14	Trp 14	engineered mutation
Ala 337	Glu 337	engineered mutation
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	101787.6
All*	Total formula weight	101787.6

*Water molecules are not included.