6NL7

Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Immunoglobulin G-binding protein G	polymer	56	6247.9	4	UniProt (P19909) Pfam (PF01378) In PDB	Streptococcus	IgG-binding protein G
2	A, B	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A, B, C, D	ACETATE ION	non-polymer		59.0	8	Chemie (ACT)
4	A, B, C	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	B	DIPHOSPHATE	non-polymer		173.9	1	Chemie (DPO)
8		water	water		18.0	335	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 56 (UniProt: P19909)

PDB	External Database	Details
Met 1	-	initiating methionine
Phe 16	Thr 317	engineered mutation
Ala 18	Thr 319	engineered mutation
His 21	Val 322	engineered mutation
His 25	Thr 326	engineered mutation
Tyr 28	Lys 329	engineered mutation
Ile 29	Val 330	engineered mutation
Arg 31	Lys 332	engineered mutation
Ala 32	Gln 333	engineered mutation
Leu 33	Tyr 334	engineered mutation
Lys 35	Asn 336	engineered mutation
Ala 36	Asp 337	engineered mutation
Gln 37	Asn 338	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	24991.8
Non-Polymers*	Number of molecules	20
	Total formula weight	1225.3
All*	Total formula weight	26217.1

*Water molecules are not included.