6M9T

Crystal structure of EP3 receptor bound to misoprostol-FA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Prostaglandin E2 receptor EP3 subtype, Endolysin chimera	polymer	537	60023.4	1	UniProt (P43115) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) In PDB	Homo sapiens (Human)	PGE2 receptor EP3 subtype,PGE2-R,Prostanoid EP3 receptor,Lysis protein,Lysozyme,Muramidase
2	A	(11alpha,12alpha,13E,16S)-11,16-dihydroxy-16-methyl-9-oxoprost-13-en-1-oic acid	non-polymer		368.5	1	Chemie (J9P)
3	A	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	1	Chemie (OLC)
5	A	OLEIC ACID	non-polymer		282.5	4	Chemie (OLA)
6		water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 2 - 259 (UniProt: P43115)

PDB	External Database	Details
Val -11	-	expression tag
Phe -10	-	expression tag
Ala -9	-	expression tag
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag

A: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1012	Arg 12	variant
Thr 1054	Cys 54	engineered mutation
Thr 1074	Ala 74	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	variant
Gly 1162	-	linker
Ser 1163	-	linker

A: 273 - 353 (UniProt: P43115)

PDB	External Database	Details
Gly 1000	-	linker
Ser 1001	-	linker
Ala 286	Gly 286	engineered mutation
Gly 2000	-	expression tag
Arg 2001	-	expression tag
Pro 2002	-	expression tag
Leu 2003	-	expression tag
Glu 2004	-	expression tag
Val 2005	-	expression tag
Leu 2006	-	expression tag
Phe 2007	-	expression tag
Gln 2008	-	expression tag
Gly 2009	-	expression tag
Pro 2010	-	expression tag
His 2011	-	expression tag
His 2012	-	expression tag
His 2013	-	expression tag
His 2014	-	expression tag
His 2015	-	expression tag
His 2016	-	expression tag
His 2017	-	expression tag
His 2018	-	expression tag
His 2019	-	expression tag
His 2020	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	60023.4
Non-Polymers*	Number of molecules	10
	Total formula weight	2239.1
All*	Total formula weight	62262.6

*Water molecules are not included.