6LTJ

Structure of nucleosome-bound human BAF complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.3	polymer	136	15361.0	2	UniProt (Q6PI79) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
2	B, F	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Histone H2A type 1	polymer	130	13993.3	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H	Histone H2B	polymer	126	13873.1	2	UniProt (Q92131) Pfam (PF00125) UniProt (by SIFTS) (P02281) In PDB	Xenopus laevis (African clawed frog)
5	I	Transcription activator BRG1	polymer	1647	184923.8	1	UniProt (P51532) Pfam (PF00271) Pfam (PF14619) Pfam (PF00439) Pfam (PF08880) Pfam (PF07529) Pfam (PF07533) Pfam (PF00176) In PDB	Homo sapiens (Human)	ATP-dependent helicase SMARCA4,BRG1-associated factor 190A,BAF190A
6	J	Actin-like protein 6A	polymer	407	45236.3	1	UniProt (O96019) Pfam (PF00022) In PDB	Homo sapiens (Human)	Actin-related protein Baf53a,ArpNbeta,BRG1-associated factor 53A,BAF53A
7	K	Actin, cytoplasmic 1	polymer	375	41782.7	1	UniProt (P60709) Pfam (PF00022) In PDB	Homo sapiens (Human)	Beta-actin
8	L	AT-rich interactive domain-containing protein 1A	polymer	1295	142316.5	1	UniProt (O14497) Pfam (PF01388) Pfam (PF12031) In PDB	Homo sapiens (Human)	ARID domain-containing protein 1A,B120,BRG1-associated factor 250,BAF250,BRG1-associated factor 250a,BAF250A
9	M	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1	polymer	336	38015.1	1	UniProt (Q12824) Pfam (PF21459) Pfam (PF04855) In PDB	Homo sapiens (Human)	BRG1-associated factor 47,BAF47
10	N, O	SWI/SNF complex subunit SMARCC2	polymer	1214	133048.1	2	UniProt (Q8TAQ2) Pfam (PF16496) Pfam (PF04433) Pfam (PF00249) Pfam (PF16498) Pfam (PF16495) In PDB	Homo sapiens (Human)	BRG1-associated factor 170,BAF170
11	P	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1	polymer	515	58311.4	1	UniProt (Q96GM5) Pfam (PF02201) In PDB	Homo sapiens (Human)
12	Q	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1	polymer	411	46710.4	1	UniProt (Q969G3) Pfam (PF00505) In PDB	Homo sapiens (Human)
13	R	Zinc finger protein ubi-d4	polymer	292	32781.9	1	UniProt (Q92785) Pfam (PF14051) Pfam (PF00628) In PDB	Homo sapiens (Human)	Apoptosis response zinc finger protein,BRG1-associated factor 45D,BAF45D
14	X	DNA (119-MER)	polymer	119	36520.3	1		Homo sapiens
15	Y	DNA (119-MER)	polymer	119	36929.5	1		Homo sapiens
16	L	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

J: 12 - 417 (UniProt: O96019)

PDB	External Database	Details
Pro 418	-	expression tag

M: 1 - 162 (UniProt: Q12824)
M: 172 - 385 (UniProt: Q12824)

PDB	External Database	Details
Gly 163	-	linker
Gly 164	-	linker
Ser 165	-	linker
Gly 166	-	linker
Gly 167	-	linker
Ser 168	-	linker
Gly 169	-	linker
Gly 170	-	linker
Ser 171	-	linker

R: 1 - 100 (UniProt: Q92785)
R: 110 - 292 (UniProt: Q92785)

PDB	External Database	Details
Gly 101	-	linker
Gly 102	-	linker
Ser 103	-	linker
Gly 104	-	linker
Gly 105	-	linker
Ser 106	-	linker
Gly 107	-	linker
Gly 108	-	linker
Ser 109	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	1038867.7
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	1038933.1

*Water molecules are not included.