6KIU
Cryo-EM structure of human MLL1-ubNCP complex (3.2 angstrom)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E | Histone H3 | polymer | 135 | 15303.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) In PDB | Xenopus laevis (African clawed frog) | |
| 2 | B, F | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) In PDB | Xenopus laevis (African clawed frog) | |
| 3 | C, G | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) In PDB | Xenopus laevis (African clawed frog) | |
| 4 | D, H | Histone H2B 1.1 | polymer | 122 | 13498.7 | 2 | UniProt (P02281) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I | DNA (145-MER) | polymer | 145 | 44521.4 | 1 | synthetic construct | ||
| 6 | J | DNA (145-MER) | polymer | 145 | 44992.6 | 1 | synthetic construct | ||
| 7 | K | Histone-lysine N-methyltransferase 2A | polymer | 216 | 24970.5 | 1 | UniProt (Q03164) Pfam (PF00856) In PDB | Homo sapiens (Human) | Lysine N-methyltransferase 2A,ALL-1,CXXC-type zinc finger protein 7,Myeloid/lymphoid or mixed-lineage leukemia,Myeloid/lymphoid or mixed-lineage leukemia protein 1,Trithorax-like protein,Zinc finger protein HRX |
| 8 | N | Retinoblastoma-binding protein 5 | polymer | 538 | 59223.5 | 1 | UniProt (Q15291) Pfam (PF00400) In PDB | Homo sapiens (Human) | RBBP-5,Retinoblastoma-binding protein RBQ-3 |
| 9 | R | WD repeat-containing protein 5 | polymer | 334 | 36635.4 | 1 | UniProt (P61964) Pfam (PF00400) In PDB | Homo sapiens (Human) | BMP2-induced 3-kb gene protein |
| 10 | T | Set1/Ash2 histone methyltransferase complex subunit ASH2 | polymer | 534 | 60288.8 | 1 | UniProt (Q9UBL3) Pfam (PF21198) Pfam (PF00622) In PDB | Homo sapiens (Human) | ASH2-like protein |
| 11 | O | Ubiquitin | polymer | 76 | 8622.9 | 1 | UniProt (P62979) Pfam (PF00240) In PDB | Homo sapiens (Human) | |
| 12 | K | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
| 13 | K | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 14 | K | LYSINE | non-polymer | 147.2 | 1 | Chemie (LYS) | |||
| 15 | K | GLUTAMINE | non-polymer | 146.1 | 1 | Chemie (GLN) |
Sequence modifications
D, H: 1 - 122 (UniProt: P02281)
O: 1 - 76 (UniProt: P62979)
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | engineered mutation |
| Cys 117 | Lys 121 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 76 | Gly 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 387343.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 743.2 | |
| All* | Total formula weight | 388086.5 |
