6J99

Cryo-EM structure of human DOT1L in complex with an H2B-monoubiquitinated nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3	polymer	136	15435.1	2	UniProt (A0A310TTQ1) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
2	B, F	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Histone H2A	polymer	130	14109.4	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H	Histone H2B 1.1	polymer	123	13629.9	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I	DNA (144-MER)	polymer	147	45138.8	1		synthetic construct
6	J	DNA (145-MER)	polymer	147	45610.0	1		synthetic construct
7	L	Ubiquitin	polymer	76	8622.9	1	UniProt (P62979) Pfam (PF00240) In PDB	Homo sapiens (Human)
8	K	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	416	47506.2	1	UniProt (Q8TEK3) Pfam (PF08123) In PDB	Homo sapiens (Human)	DOT1-like protein,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4
9	K	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)

Sequence modifications

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	engineered mutation
Cys 117	Lys 121	engineered mutation

L: 1 - 76 (UniProt: P62979)

PDB	External Database	Details
Cys 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	256015.7
Non-Polymers*	Number of molecules	1
	Total formula weight	384.4
All*	Total formula weight	256400.1

*Water molecules are not included.