6IX2

Structure of the A214C/A250I mutant of an epoxide hydrolase from Aspergillus usamii E001 (AuEH2) at 1.48 Angstroms resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Microsomal epoxide hyddrolase	polymer	431	48570.8	2	UniProt (T2B4K5) Pfam (PF06441) In PDB	Aspergillus usamii
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3		water	water		18.0	1041	Chemie (HOH)

Sequence modifications

A, B: 1 - 395 (UniProt: T2B4K5)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Cys 214	Ala 214	engineered mutation
Ile 250	Ala 250	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97141.6
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	97233.7

*Water molecules are not included.