6IJ6

Crystal structure of PETase S121E, D186H, R280A mutant from Ideonella sakaiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Poly(ethylene terephthalate) hydrolase	polymer	300	31498.0	1	UniProt (A0A0K8P6T7) Pfam (PF01738) In PDB	Ideonella sakaiensis	PETase
2		water	water		18.0	188	Chemie (HOH)

Sequence modifications

A: 34 - 290 (UniProt: A0A0K8P6T7)

PDB	External Database	Details
Met 13	-	initiating methionine
Gly 14	-	expression tag
Ser 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
Ser 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
Leu 26	-	expression tag
Val 27	-	expression tag
Pro 28	-	expression tag
Arg 29	-	expression tag
Gly 30	-	expression tag
Ser 31	-	expression tag
His 32	-	expression tag
Met 33	-	expression tag
Glu 121	Ser 121	engineered mutation
His 186	Asp 186	engineered mutation
Ala 280	Arg 280	engineered mutation
Leu 291	-	expression tag
Glu 292	-	expression tag
Asp 293	-	expression tag
Pro 294	-	expression tag
Ala 295	-	expression tag
Ala 296	-	expression tag
Asn 297	-	expression tag
Lys 298	-	expression tag
Ala 299	-	expression tag
Arg 300	-	expression tag
Lys 301	-	expression tag
Glu 302	-	expression tag
Ala 303	-	expression tag
Arg 304	-	expression tag
Leu 305	-	expression tag
Ala 306	-	expression tag
Ala 307	-	expression tag
Ala 308	-	expression tag
Thr 309	-	expression tag
Ala 310	-	expression tag
Glu 311	-	expression tag
Gln 312	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31498.0
All*	Total formula weight	31498.0

*Water molecules are not included.