6IDO

Crystal structure of Klebsiella pneumoniae sigma4 of sigmaS fusing with the RNA polymerase beta-flap-tip-helix in complex with -35 element DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RNA polymerase sigma factor RpoS,RNA polymerase beta-flap-tip-helix	polymer	115	12946.7	2	UniProt (Q9F8R5) Pfam (PF04545) UniProt (by SIFTS) (P13445) UniProt (by SIFTS) (A6TGP0) In PDB	Escherichia coli	Sigma S,Sigma S
2	C, E	DNA (5'-D(P*GP*AP*TP*TP*TP*GP*TP*CP*AP*AP*GP*TP*GP*GP*C)-3')	polymer	15	4640.0	2		Klebsiella pneumoniae
3	D, F	DNA (5'-D(P*CP*CP*AP*CP*TP*TP*GP*AP*CP*AP*AP*AP*TP*CP*G)-3')	polymer	16	4867.2	2		Klebsiella pneumoniae

Sequence modifications

A, B: 16 - 90 (UniProt: Q9F8R5)

PDB	External Database	Details
Gly 1	-	expression tag
Ala 2	-	expression tag
Met 3	-	expression tag
Gly 4	-	expression tag
Gly 5	-	expression tag
Pro 6	-	expression tag
Glu 7	-	expression tag
Asp 8	-	expression tag
Thr 9	-	expression tag
Thr 10	-	expression tag
Gln 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Met 15	-	expression tag
Gly 79	Thr 319	engineered mutation
Gly 91	-	linker
Ser 92	-	linker
Gly 93	-	linker
Ser 94	-	linker

A, B: 95 - 115 (PDB: 6IDO)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	44907.7
All*	Total formula weight	44907.7

*Water molecules are not included.