6DDZ

Crystal structure of the double mutant (D52N/R238W) of NT5C2-537X in the active state, Northeast Structural Genomics Target

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytosolic purine 5'-nucleotidase	polymer	554	63985.8	1	UniProt (P49902) Pfam (PF05761) In PDB	Homo sapiens (Human)	Cytosolic 5'-nucleotidase II
2	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	342	Chemie (HOH)

Sequence modifications

A: 1 - 536 (UniProt: P49902)

PDB	External Database	Details
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 52	Asp 52	engineered mutation
Trp 238	Arg 238	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	63985.8
Non-Polymers*	Number of molecules	6
	Total formula weight	834.9
All*	Total formula weight	64820.7

*Water molecules are not included.