6ANV

Crystal structure of anti-CRISPR protein AcrF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	anti-CRISPR protein AcrF1 fused with C-terminal MBP tag	polymer	452	49773.1	2	UniProt (L7P7M1) UniProt (P0AEY0) Pfam (PF20829) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Pseudomonas phage JBD30	MBP,MMBP,Maltodextrin-binding protein
2	C, D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		666.6	2	In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ)		alpha-maltotetraose
3	A, B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
4	A, B	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
5	A, B	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
6	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	7	Chemie (PEG)
7		water	water		18.0	253	Chemie (HOH)

Sequence modifications

A, B: 1 - 78 (UniProt: L7P7M1)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Leu 79	-	linker
Glu 80	-	linker

A, B: 81 - 450 (UniProt: P0AEY0)

PDB	External Database	Details
Asn 447	Arg 393	engineered mutation
Ala 448	Ile 394	engineered mutation
Ala 449	Thr 395	engineered mutation
Ala 450	Lys 396	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99546.3
Branched	Number of molecules	2
	Total formula weight	1333.2
Non-Polymers*	Number of molecules	19
	Total formula weight	2018.3
All*	Total formula weight	102897.7

*Water molecules are not included.