6A8H

Crystal structure of endo-arabinanase ABN-TS D27A mutant in complex with arabinotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	endo-alpha-(1->5)-L-arabinanase	polymer	321	36514.7	1	UniProt (Q93HT9) Pfam (PF04616) In PDB	Geobacillus thermodenitrificans	arabinanase-TS,ABN,Endo-1,5-alpha-L-arabinanase
2	B, C	alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose	branched		414.4	2	In PDB GlyTouCan (G56809YT)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5		water	water		18.0	266	Chemie (HOH)

Sequence modifications

A: 1 - 313 (UniProt: Q93HT9)

PDB	External Database	Details
Ala 27	Asp 27	engineered mutation
Leu 314	-	expression tag
Glu 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36514.7
Branched	Number of molecules	2
	Total formula weight	828.7
Non-Polymers*	Number of molecules	2
	Total formula weight	64.4
All*	Total formula weight	37407.8

*Water molecules are not included.