5Z02

Crystal structure of HIS6-tagged Mdm2 with nutlin-3a

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 ubiquitin-protein ligase Mdm2	polymer	110	12956.9	1	UniProt (Q00987) Pfam (PF02201) In PDB	Homo sapiens (Human)	Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
2	A	4-({(4S,5R)-4,5-bis(4-chlorophenyl)-2-[4-methoxy-2-(propan-2-yloxy)phenyl]-4,5-dihydro-1H-imidazol-1-yl}carbonyl)piperazin-2-one	non-polymer		581.5	1	Chemie (NUT)
3		water	water		18.0	33	Chemie (HOH)

Sequence modifications

A: 3 - 91 (UniProt: Q00987)

PDB	External Database	Details
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Gln -8	-	expression tag
Asp -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	12956.9
Non-Polymers*	Number of molecules	1
	Total formula weight	581.5
All*	Total formula weight	13538.4

*Water molecules are not included.