5WMM
Crystal structure of an adenylation domain interrupted by a methylation domain (AMA4) from nonribosomal peptide synthetase TioS
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | NRPS | polymer | 926 | 102016.6 | 1 | UniProt (Q333U7) Pfam (PF00501) Pfam (PF08242) In PDB | Micromonospora sp. ML1 | |
| 2 | B | MbtH homologue | polymer | 87 | 9891.7 | 1 | UniProt (Q333U6) Pfam (PF03621) In PDB | Micromonospora sp. ML1 | MbtH-like protein TioT |
| 3 | A | (2S)-2-amino-3-methylbutanoyl (2S,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl hydrogen (S)-phosphate | non-polymer | 432.3 | 1 | Chemie (B6G) | |||
| 4 | A | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
| 5 | A | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 6 | A | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 7 | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A: 1 - 905 (UniProt: Q333U7)
B: 1 - 71 (UniProt: Q333U6)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Ser -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 111908.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1043.3 | |
| All* | Total formula weight | 112951.6 |
