5W5M

Crystal structure of human IgG4-Sigma1 Fc fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Immunoglobulin heavy constant gamma 4	polymer	223	25030.2	2	UniProt (P01861) Pfam (PF07654) In PDB	Homo sapiens (Human)	Ig gamma-4 chain C region
2	C, D	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1625.5	2	In PDB GlyTouCan (G27919IH)
3	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	B	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5		water	water		18.0	215	Chemie (HOH)

Sequence modifications

A, B: 226 - 447 (UniProt: P01861)

PDB	External Database	Details
Thr 225	-	expression tag
Pro 228	Ser 108	engineered mutation
Ala 234	Phe 114	engineered mutation
Ala 235	Leu 115	engineered mutation
Ala 237	Gly 117	engineered mutation
Ser 238	Pro 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50060.3
Branched	Number of molecules	2
	Total formula weight	3251.0
Non-Polymers*	Number of molecules	3
	Total formula weight	243.2
All*	Total formula weight	53554.6

*Water molecules are not included.