5VZD

Pre-catalytic ternary complex of human Polymerase Mu (W434A) mutant with incoming nonhydrolyzable UMPNPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed DNA/RNA polymerase mu	polymer	354	39729.0	1	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (Human)	Pol Mu,Terminal transferase
2	T	DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3')	polymer	9	2740.8	1		synthetic construct
3	P	DNA (5'-D(*CP*GP*TP*A)-3')	polymer	4	1190.8	1		synthetic construct
4	D	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	1		synthetic construct
5	A	5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine	non-polymer		483.2	1	Chemie (2KH)
6	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
9	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
10		water	water		18.0	378	Chemie (HOH)

Sequence modifications

A: 134 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
Gly 129	-	expression tag
Ser 130	-	expression tag
Ala 131	-	expression tag
Ala 132	-	expression tag
Ala 133	-	expression tag
-	Pro 398	deletion
-	Gly 399	deletion
-	Ala 400	deletion
-	Ala 401	deletion
-	Val 402	deletion
-	Gly 403	deletion
-	Gly 404	deletion
-	Ser 405	deletion
-	Thr 406	deletion
-	Arg 407	deletion
-	Pro 408	deletion
-	Cys 409	deletion
Gly 410	Pro 410	linker
Ala 434	Trp 434	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	44852.5
Non-Polymers*	Number of molecules	7
	Total formula weight	917.2
All*	Total formula weight	45769.7

*Water molecules are not included.