5VZC

Post-catalytic complex of human Polymerase Mu (G433S) mutant with incoming dTTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed DNA/RNA polymerase mu	polymer	354	39874.2	1	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (Human)	Pol Mu,Terminal transferase
2	T	DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3')	polymer	9	2740.8	1		synthetic construct
3	P	DNA (5'-D(*CP*GP*TP*AP*T)-3')	polymer	5	1495.0	1		synthetic construct
4	D	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	1		synthetic construct
5	A, P	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
8	A	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	1	Chemie (TTP)
9	A	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
10	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
11		water	water		18.0	441	Chemie (HOH)

Sequence modifications

A: 134 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
Gly 129	-	expression tag
Ser 130	-	expression tag
Ala 131	-	expression tag
Ala 132	-	expression tag
Ala 133	-	expression tag
-	Pro 398	deletion
-	Gly 399	deletion
-	Ala 400	deletion
-	Ala 401	deletion
-	Val 402	deletion
-	Gly 403	deletion
-	Gly 404	deletion
-	Ser 405	deletion
-	Thr 406	deletion
-	Arg 407	deletion
-	Pro 408	deletion
-	Cys 409	deletion
Gly 410	Pro 410	linker
Ser 433	Gly 433	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45301.8
Non-Polymers*	Number of molecules	11
	Total formula weight	952.6
All*	Total formula weight	46254.4

*Water molecules are not included.