5VMF

Influenza hemagglutinin H1 mutant DH1D in complex with 6'SLN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Hemagglutinin HA1	polymer	326	35998.3	3	UniProt (Q9WFX3) Pfam (PF00509) In PDB	Influenza A virus (strain A/Brevig Mission/1/1918 H1N1)
2	B, D, F	Hemagglutinin HA2	polymer	191	21725.1	3	UniProt (Q9WFX3) Pfam (PF00509) In PDB	Influenza A virus (strain A/Brevig Mission/1/1918 H1N1)
3	G	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	H, I, K	N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	3	In PDB BIRD (PRD_900046) GlyTouCan (G73578JC)		6'-sialyl-N-acetyllactosamine
5	J	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
6	A, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7		water	water		18.0	900	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 326 (UniProt: Q9WFX3)

PDB	External Database	Details
Leu 222	Gln 240	engineered mutation
Ser 224	Gly 242	engineered mutation

B, D, F: 1 - 185 (UniProt: Q9WFX3)

PDB	External Database	Details
Gly 186	-	expression tag
Ala 187	-	expression tag
Leu 188	-	expression tag
Val 189	-	expression tag
Pro 190	-	expression tag
Arg 191	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	173170.2
Branched	Number of molecules	5
	Total formula weight	3034.8
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	176868.6

*Water molecules are not included.