5TMF

Re-refinement of thermus thermophilus RNA polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	2	UniProt (Q9Z9H6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (Q5SHR6) In PDB	Thermus thermophilus	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	1	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Thermus thermophilus	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	1	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Thermus thermophilus	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	99	11521.2	1	UniProt (Q72ID6) Pfam (PF01192) UniProt (by SIFTS) (Q8RQE7) In PDB	Thermus thermophilus	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor SigA	polymer	423	48598.0	1	UniProt (Q72L95) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (Q5SKW1) In PDB	Thermus thermophilus
6	A, D	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	C	methyl [(1E,5R)-5-{(3S)-3-[(2E,4E)-2,5-dimethylocta-2,4-dienoyl]-2,4-dioxo-3,4-dihydro-2H-pyran-6-yl}hexylidene]carbamate	non-polymer		417.5	1	Chemie (NE6)
8	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
9	D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
10		water	water		18.0	49	Chemie (HOH)

Sequence modifications

E: 1 - 99 (UniProt: Q72ID6)

PDB	External Database	Details
Glu 61	Val 61	conflict
Ile 92	Leu 92	conflict
Gly 95	Val 95	conflict

F: 1 - 423 (UniProt: Q72L95)

PDB	External Database	Details
Thr 46	Ala 46	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	426665.5
Non-Polymers*	Number of molecules	7
	Total formula weight	786.9
All*	Total formula weight	427452.4

*Water molecules are not included.