5T8S

Crystal Structure of a S-adenosylmethionine Synthase from Neisseria gonorrhoeae with bound S-adenosylmethionine, AMP, Pyrophosphate, Phosphate, and Magnesium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	S-adenosylmethionine synthase	polymer	397	43017.5	2	UniProt (Q5FAC0) Pfam (PF00438) Pfam (PF02772) Pfam (PF02773) In PDB	Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)	AdoMet synthase,MAT,Methionine adenosyltransferase
2	A, B	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	A	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
4	A	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
5	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	B	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
7	B	TRIPHOSPHATE	non-polymer		258.0	1	Chemie (3PO)
8		water	water		18.0	688	Chemie (HOH)

Sequence modifications

A, B: 1 - 389 (UniProt: Q5FAC0)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	86035.0
Non-Polymers*	Number of molecules	9
	Total formula weight	1371.8
All*	Total formula weight	87406.7

*Water molecules are not included.