5MIO
KIF2C-DARPIN FUSION PROTEIN BOUND TO TUBULIN
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 1 | UniProt (P81947) Pfam (PF00091) Pfam (PF03953) In PDB | Bos taurus (cattle) | Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain |
| 2 | B | Tubulin beta chain | polymer | 445 | 49999.9 | 1 | UniProt (D0VWY9) Pfam (PF00091) Pfam (PF03953) In PDB | Ovis aries (Sheep) | |
| 3 | C | Kinesin-like protein KIF2C,KIF2C FUSED TO A DARPIN,KIF2C FUSED TO A DARPIN | polymer | 573 | 62110.4 | 1 | UniProt (Q99661) Pfam (PF00225) In PDB | Homo sapiens (Human) | Kinesin-like protein 6,Mitotic centromere-associated kinesin,MCAK |
| 4 | A | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 5 | A, C | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | B | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 7 | B | N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide | non-polymer | 399.4 | 1 | Chemie (LOC) | |||
| 8 | C | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) |
Sequence modifications
B: 1 - 455 (UniProt: D0VWY9)
C: 216 - 598 (UniProt: Q99661)
C: 622 - 640 (PDB: 5MIO)
C: 641 - 786 (PDB: 5MIO)
| PDB | External Database | Details |
|---|---|---|
| Cys 203 | Ser 201 | conflict |
| Ile 318 | Val 316 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 214 | - | initiating methionine |
| Gly 215 | - | expression tag |
| Ala 330 | Arg 330 | engineered mutation |
| Ala 379 | Arg 379 | engineered mutation |
| Gly 599 | - | linker |
| Gly 600 | - | linker |
| Gly 601 | - | linker |
| Gly 602 | - | linker |
| Ser 603 | - | linker |
| Gly 604 | - | linker |
| Gly 605 | - | linker |
| Gly 606 | - | linker |
| Gly 607 | - | linker |
| Ser 608 | - | linker |
| Gly 609 | - | linker |
| Gly 610 | - | linker |
| Gly 611 | - | linker |
| Gly 612 | - | linker |
| Ser 613 | - | linker |
| Gly 614 | - | linker |
| Gly 615 | - | linker |
| Gly 616 | - | linker |
| Gly 617 | - | linker |
| Ser 618 | - | linker |
| Gly 619 | - | linker |
| Gly 620 | - | linker |
| Ser 621 | - | linker |
C: 641 - 786 (PDB: 5MIO)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 162314.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1920.6 | |
| All* | Total formula weight | 164235.4 |
