5M7C

Blood group synthase AAGlyB in complex with UDP and cryoprotected with PEG 3350

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Histo-blood group ABO system transferase	polymer	298	34654.0	2	UniProt (P16442) Pfam (PF03414) In PDB	Homo sapiens (Human)	Fucosylglycoprotein 3-alpha-galactosyltransferase,Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase,Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase,Glycoprotein-fucosylgalactoside alpha-galactosyltransferase,Histo-blood group A transferase,A transferase,Histo-blood group B transferase,B transferase,NAGAT
2	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	A, B	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6		water	water		18.0	374	Chemie (HOH)

Sequence modifications

A, B: 64 - 354 (UniProt: P16442)

PDB	External Database	Details
Met 57	-	expression tag
Ala 58	-	expression tag
Ile 59	-	expression tag
Gly 60	-	expression tag
Glu 61	-	expression tag
Phe 62	-	expression tag
Met 63	-	expression tag
Gly 266	Leu 266	engineered mutation
Ala 268	Gly 268	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69308.0
Non-Polymers*	Number of molecules	8
	Total formula weight	1322.6
All*	Total formula weight	70630.6

*Water molecules are not included.