5FGU

Structure of Sda1 nuclease apoprotein as an EGFP fixed-arm fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein,Extracellular streptodornase D	polymer	567	63627.6	1	UniProt (P42212) UniProt (Q675N6) Pfam (PF01353) Pfam (PF01223) In PDB	Aequorea victoria (Jellyfish)	Phage-encoded extracellular streptodornase D,Phage-encoded streptodornase,Streptodornase
2	A	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
3	A	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
4	A	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
5		water	water		18.0	327	Chemie (HOH)

Sequence modifications

A: 1 - 229 (UniProt: P42212)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
-	Ser 65	chromophore
-	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Ala 72	Ser 72	engineered mutation
Ala 230	-	linker
Ala 231	-	linker
Ala 232	-	linker

A: 1059 - 1390 (UniProt: Q675N6)

PDB	External Database	Details
Gly 1188	His 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	63627.6
Non-Polymers*	Number of molecules	14
	Total formula weight	1060.8
All*	Total formula weight	64688.5

*Water molecules are not included.