5E2J

Crystal structure of single mutant thermostable endoglucanase (D468A) from Alicyclobacillus acidocaldarius

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Endoglucanase	polymer	570	62834.3	2	UniProt (Q9AJS0) Pfam (PF02927) Pfam (PF00759) In PDB	Alicyclobacillus acidocaldarius
2	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, B	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	3	Chemie (MPD)
5		water	water		18.0	398	Chemie (HOH)

Sequence modifications

A, B: 1 - 537 (UniProt: Q9AJS0)

PDB	External Database	Details
Met -22	-	expression tag
Ala -21	-	expression tag
Ser -20	-	expression tag
Met -19	-	expression tag
Thr -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Gln -15	-	expression tag
Gln -14	-	expression tag
Met -13	-	expression tag
Gly -12	-	expression tag
Arg -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Glu -8	-	expression tag
Phe -7	-	expression tag
Glu -6	-	expression tag
Leu -5	-	expression tag
Arg -4	-	expression tag
Arg -3	-	expression tag
Arg -2	-	expression tag
Phe -1	-	expression tag
Asp 0	-	expression tag
Ala 468	Asp 468	engineered mutation
Ala 538	-	expression tag
Ala 539	-	expression tag
Leu 540	-	expression tag
Glu 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag
His 547	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	125668.6
Non-Polymers*	Number of molecules	7
	Total formula weight	565.5
All*	Total formula weight	126234.1

*Water molecules are not included.