5D0G

Crystal structure of triple mutant (KDA to EGY) of adenylyl cyclase Ma1120 from Mycobacterium avium in complex with GTP and calcium ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cyclase	polymer	177	19621.3	2	UniProt (V7LAR8) In PDB	Mycobacterium avium subsp. avium 10-9275
2	A, B	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4		water	water		18.0	224	Chemie (HOH)

Sequence modifications

A, B: 106 - 275 (UniProt: V7LAR8)

PDB	External Database	Details
Phe 99	-	expression tag
Gln 100	-	expression tag
Gly 101	-	expression tag
Ala 102	-	expression tag
Met 103	-	expression tag
Gly 104	-	expression tag
Ser 105	-	expression tag
Glu 153	Lys 153	engineered mutation
Gly 209	Asp 209	engineered mutation
Tyr 219	Ala 219	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39242.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1126.5
All*	Total formula weight	40369.2

*Water molecules are not included.