5BUZ

Crystal Structure of a Complex Between the SNARE Vam3 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	SM (Sec1/Munc18-like) protein	polymer	669	73990.1	2	UniProt (G0SCM5) Pfam (PF00995) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	VPS33
2	B, E	Putative vacuolar protein sorting-associated protein	polymer	333	37912.2	2	UniProt (G0S6M7) Pfam (PF04840) In PDB	Chaetomium thermophilum	Vps16
3	C, F	SNAP receptor-like protein	polymer	67	7504.2	2	UniProt (G0S236) Pfam (PF05739) In PDB	Chaetomium thermophilum	VAM3

Sequence modifications

A, D: 0 - 667 (UniProt: G0SCM5)

PDB	External Database	Details
Gly -1	-	expression tag

B, E: 505 - 834 (UniProt: G0S6M7)

PDB	External Database	Details
Met 502	-	initiating methionine
Gly 503	-	expression tag
Ser 504	-	expression tag
Arg 672	-	insertion
Met 673	-	insertion
Gln 674	-	insertion
Glu 675	-	insertion
Thr 676	-	insertion
Phe 677	-	insertion
Glu 678	-	insertion
Arg 679	-	insertion
Asp 680	-	insertion
Leu 681	-	insertion
Thr 682	-	insertion
Asp 683	-	insertion
Ser 684	-	insertion
Phe 685	-	insertion
Val 686	-	insertion
Gly 687	-	insertion
Leu 688	-	insertion
Ser 689	-	insertion
Val 690	Leu 672	engineered mutation

C, F: 181 - 245 (UniProt: G0S236)

PDB	External Database	Details
Gly 179	-	expression tag
Ser 180	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	238813.0
All*	Total formula weight	238813.0

*Water molecules are not included.