5BR8

Ambient-temperature crystal structure of 30S ribosomal subunit from Thermus thermophilus in complex with paromomycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	16S ribosomal RNA	polymer	1522	494169.8	1	GenBank (55771382)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
2	B	30S ribosomal protein S2	polymer	256	29317.7	1	UniProt (P80371) Pfam (PF00318) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
3	C	30S ribosomal protein S3	polymer	239	26751.1	1	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
4	D	30S ribosomal protein S4	polymer	209	24373.4	1	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
5	E	30S ribosomal protein S5	polymer	162	17583.4	1	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
6	F	30S ribosomal protein S6	polymer	101	11988.8	1	UniProt (Q5SLP8) Pfam (PF01250) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TS9
7	G	30S ribosomal protein S7	polymer	156	18051.0	1	UniProt (P17291) Pfam (PF00177) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
8	H	30S ribosomal protein S8	polymer	138	15868.6	1	UniProt (Q5SHQ2) Pfam (PF00410) UniProt (by SIFTS) (P0DOY9) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
9	I	30S ribosomal protein S9	polymer	128	14410.6	1	UniProt (P80374) Pfam (PF00380) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
10	J	30S ribosomal protein S10	polymer	105	11955.0	1	UniProt (Q5SHN7) Pfam (PF00338) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
11	K	30S ribosomal protein S11	polymer	129	13737.9	1	UniProt (P80376) Pfam (PF00411) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
12	L	30S ribosomal protein S12	polymer	135	14966.8	1	UniProt (Q5SHN3) Pfam (PF00164) In PDB	Thermus thermophilus
13	M	30S ribosomal protein S13	polymer	126	14338.9	1	UniProt (P80377) Pfam (PF00416) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
14	N	30S ribosomal protein S14 type Z	polymer	61	7158.7	1	UniProt (Q5SHQ1) Pfam (PF00253) UniProt (by SIFTS) (P0DOY6) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
15	O	30S ribosomal protein S15	polymer	89	10578.4	1	UniProt (Q5SJ76) Pfam (PF00312) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
16	P	30S ribosomal protein S16	polymer	88	10410.0	1	UniProt (Q5SJH3) Pfam (PF00886) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
17	Q	30S ribosomal protein S17	polymer	105	12324.7	1	UniProt (Q5SHP7) Pfam (PF00366) UniProt (by SIFTS) (P0DOY7) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
18	R	30S ribosomal protein S18	polymer	88	10258.3	1	UniProt (Q5SLQ0) Pfam (PF01084) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
19	S	30S ribosomal protein S19	polymer	93	10605.5	1	UniProt (Q5SHP2) Pfam (PF00203) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
20	T	30S ribosomal protein S20	polymer	106	11736.1	1	UniProt (P80380) Pfam (PF01649) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
21	U	30S ribosomal protein Thx	polymer	27	3350.0	1	UniProt (Q5SIH3) Pfam (PF17070) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	S31
22	A	PAROMOMYCIN	non-polymer		615.6	6	Chemie (PAR)
23	A, S, T, B, D...	MAGNESIUM ION	non-polymer		24.3	287	Chemie (MG)
24	D, N	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
25		water	water		18.0	308	Chemie (HOH)

Sequence modifications

A: 0 - 1533 (GenBank: 55771382)

PDB	External Database	Details
C 1534	-	expression tag
A 1535	-	expression tag
C 1536	-	expression tag
U 1537	-	expression tag
C 1538	-	expression tag
C 1539	-	expression tag
Psu 1540	-	expression tag
Psu 1541	-	expression tag
U 1542	-	expression tag
C 1543	-	expression tag
U 1544	-	expression tag

L: 5 - 135 (UniProt: Q5SHN3)

PDB	External Database	Details
Met 1	-	initiating methionine
Val 2	-	expression tag
Ala 3	-	expression tag
Leu 4	-	expression tag

Q: 1 - 105 (UniProt: Q5SHP7)

PDB	External Database	Details
Gln 96	Glu 96	conflict

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	783934.6
Non-Polymers*	Number of molecules	295
	Total formula weight	10800.1
All*	Total formula weight	794734.7

*Water molecules are not included.