5B4N

Structure analysis of function associated loop mutant of substrate recognition domain of Fbs1 ubiquitin ligase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	F-box only protein 2	polymer	203	23626.2	2	UniProt (Q80UW2) Pfam (PF04300) UniProt (by SIFTS) (Q9H4M3) In PDB	Mus musculus (Mouse)
2		water	water		18.0	93	Chemie (HOH)

Sequence modifications

A, B: 117 - 298 (UniProt: Q80UW2)

PDB	External Database	Details
Met 96	-	initiating methionine
Gly 97	-	expression tag
Ser 98	-	expression tag
Ser 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
Ser 106	-	expression tag
Ser 107	-	expression tag
Gly 108	-	expression tag
Leu 109	-	expression tag
Val 110	-	expression tag
Pro 111	-	expression tag
Arg 112	-	expression tag
Gly 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
Met 116	-	expression tag
Leu 142	Asp 142	engineered mutation
Asp 143	Val 143	engineered mutation
Val 144	Glu 144	engineered mutation
Asn 145	His 145	engineered mutation
Lys 151	Arg 151	engineered mutation
Ser 156	Pro 156	engineered mutation
Arg 157	Gly 157	engineered mutation
Gln 159	Asn 159	engineered mutation
Arg 160	Gly 160	engineered mutation
Lys 161	Val 161	engineered mutation
-	Thr 164	deletion
Pro 164	Gln 165	engineered mutation
Asn 165	Asp 166	engineered mutation
Gln 167	Ser 168	engineered mutation
Val 173	Ala 174	engineered mutation
Thr 174	Ser 175	engineered mutation
Tyr 176	Phe 177	engineered mutation
Tyr 177	Glu 178	engineered mutation
Thr 178	Trp 179	engineered mutation
Pro 214	Thr 215	engineered mutation
Cys 216	Ala 217	engineered mutation
Lys 219	Leu 220	engineered mutation
Gln 221	Glu 222	engineered mutation
Gln 238	-	insertion
Pro 239	-	insertion
Asp 240	Ala 239	engineered mutation
Pro 241	Thr 240	engineered mutation
Ala 242	Gly 241	engineered mutation
Thr 243	Gln 242	engineered mutation
Ile 244	Val 243	engineered mutation
Gln 245	Ala 244	engineered mutation
Gln 246	Val 245	engineered mutation
Lys 247	Pro 246	engineered mutation
Ser 248	Glu 247	engineered mutation
Ala 250	Gly 249	engineered mutation
Lys 251	Ser 250	engineered mutation
Arg 253	Met 252	engineered mutation
Val 276	Gln 275	engineered mutation
Thr 278	Ser 277	engineered mutation
His 279	Val 278	engineered mutation
Ala 282	Lys 281	engineered mutation
Tyr 285	Phe 284	engineered mutation
Pro 287	Ala 286	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47252.4
All*	Total formula weight	47252.4

*Water molecules are not included.