5AKQ

X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	N-ISOPROPYLAMMELIDE ISOPROPYL AMIDOHYDROLASE	polymer	423	47169.6	2	UniProt (O52063) Pfam (PF07969) In PDB	PSEUDOMONAS SP. ADP
2	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4		water	water		18.0	34	Chemie (HOH)

Sequence modifications

A, B: 1 - 403 (UniProt: O52063)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 304	Asn 304	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	94339.3
Non-Polymers*	Number of molecules	4
	Total formula weight	201.7
All*	Total formula weight	94541.0

*Water molecules are not included.