4ZK7

Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Chorismate mutase	polymer	130	14495.6	12	UniProt (Q5SJY4) Pfam (PF07736) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
2	M, N, O, P, Q...	Divalent-cation tolerance protein CutA	polymer	111	12544.4	12	UniProt (Q7SIA8) Pfam (PF03091) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 122 (UniProt: Q5SJY4)

PDB	External Database	Details
Ala 17	Glu 17	engineered mutation
Leu 20	His 20	engineered mutation
Ala 21	Gln 21	engineered mutation
Ile 24	Arg 24	engineered mutation
Leu 64	Gln 64	engineered mutation
Asn 109	Arg 109	engineered mutation
Leu 123	-	expression tag
Glu 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag

M, N, O, P, Q, R, S, T, U, V, W, X: 1 - 103 (UniProt: Q7SIA8)

PDB	External Database	Details
Ala 12	Glu 12	engineered mutation
Leu 13	Glu 13	engineered mutation
Val 16	Arg 16	engineered mutation
Lys 17	Thr 17	engineered mutation
His 20	Lys 20	engineered mutation
Glu 43	Trp 43	engineered mutation
Glu 44	Gln 44	engineered mutation
Ser 46	Glu 46	engineered mutation
Ser 49	Glu 49	engineered mutation
His 51	Gln 51	engineered mutation
Asp 62	His 62	engineered mutation
Glu 73	Ala 73	engineered mutation
Glu 78	Thr 78	engineered mutation
Leu 104	-	expression tag
Glu 105	-	expression tag
His 106	-	expression tag
His 107	-	expression tag
His 108	-	expression tag
His 109	-	expression tag
His 110	-	expression tag
His 111	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	324480.6
All*	Total formula weight	324480.6

*Water molecules are not included.