4X85
Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant H86Y/A269T/R374W
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Lipase | polymer | 391 | 43930.0 | 1 | UniProt (Q93A71) In PDB | Geobacillus stearothermophilus T6 | |
2 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
3 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
4 | water | water | 18.0 | 259 | Chemie (HOH) |
Sequence modifications
A: 5 - 389 (UniProt: Q93A71)
PDB | External Database | Details |
---|---|---|
Tyr 86 | His 115 | engineered mutation |
Thr 269 | Ala 298 | engineered mutation |
Ala 323 | Thr 352 | conflict |
Trp 374 | Arg 403 | engineered mutation |
His 390 | - | expression tag |
His 391 | - | expression tag |
His 392 | - | expression tag |
His 393 | - | expression tag |
His 394 | - | expression tag |
His 395 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 43930.0 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 105.5 | |
All* | Total formula weight | 44035.5 |