4WYR

Crystal structure of thiolase mutation (V77Q,N153Y,A286K) from Clostridium acetobutylicum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Acetyl-CoA acetyltransferase	polymer	400	42497.7	2	UniProt (P45359) Pfam (PF00108) Pfam (PF02803) In PDB	Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)	Acetoacetyl-CoA thiolase
2	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	5	Chemie (PEG)
4		water	water		18.0	261	Chemie (HOH)

Sequence modifications

A, B: 1 - 392 (UniProt: P45359)

PDB	External Database	Details
Gln 77	Val 77	engineered mutation
Tyr 153	Asn 153	engineered mutation
Lys 286	Ala 286	engineered mutation
Leu 393	-	expression tag
Glu 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84995.5
Non-Polymers*	Number of molecules	7
	Total formula weight	714.8
All*	Total formula weight	85710.2

*Water molecules are not included.