4WH4

Protein GB1 Quadruple Mutant I6H/N8H/K28H/Q32H

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Immunoglobulin G-binding protein G	polymer	56	6296.8	2	UniProt (P19909) Pfam (PF01378) In PDB	Streptococcus sp. group G	IgG-binding protein G
2	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	A, B	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4		water	water		18.0	29	Chemie (HOH)

Sequence modifications

A, B: 3 - 56 (UniProt: P19909)

PDB	External Database	Details
Met 1	-	expression tag
Gln 2	-	expression tag
His 6	Ile 307	engineered mutation
His 8	Asn 309	engineered mutation
His 28	Lys 329	engineered mutation
His 32	Gln 333	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	12593.7
Non-Polymers*	Number of molecules	6
	Total formula weight	556.5
All*	Total formula weight	13150.2

*Water molecules are not included.