4WCV

Haloalkane dehalogenase DhaA mutant from Rhodococcus rhodochrous (T148L+G171Q+A172V+C176G)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Haloalkane dehalogenase	polymer	299	34176.0	1	UniProt (P0A3G3) Pfam (PF00561) UniProt (by SIFTS) (P0A3G2) In PDB	Rhodococcus rhodochrous
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
4	A	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
5		water	water		18.0	477	Chemie (HOH)

Sequence modifications

A: 1 - 293 (UniProt: P0A3G3)

PDB	External Database	Details
Leu 148	Thr 148	engineered mutation
Gln 171	Gly 171	engineered mutation
Val 172	Ala 172	engineered mutation
Gly 176	Cys 176	engineered mutation
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34176.0
Non-Polymers*	Number of molecules	4
	Total formula weight	303.7
All*	Total formula weight	34479.7

*Water molecules are not included.