4W8B

Crystal structure of XEG5B, a GH5 xyloglucan-specific beta-1,4-glucanase from ruminal metagenomic library, in complex with XXLG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Exo-xyloglucanase	polymer	386	42681.8	1	UniProt (D2XML9) Pfam (PF00150) In PDB	uncultured bacterium
2	B	alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[beta-D-galactopyranose-(1-2)-alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		1225.1	1	In PDB GlyTouCan (G80923GO)
3	C	alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		768.7	1	In PDB GlyTouCan (G79511DD)
4	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	678	Chemie (HOH)

Sequence modifications

A: 139 - 516 (UniProt: D2XML9)

PDB	External Database	Details
Leu 517	-	expression tag
Glu 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag
His 524	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42681.8
Branched	Number of molecules	2
	Total formula weight	1993.7
Non-Polymers*	Number of molecules	5
	Total formula weight	472.4
All*	Total formula weight	45147.9

*Water molecules are not included.