4UO9

Structure of the A_Canine_Colorado_17864_06 H3 haemagglutinin Ser30Thr mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	HA1	polymer	328	36360.0	1	UniProt (E0UVR5) Pfam (PF00509) In PDB	INFLUENZA A VIRUS (A/CANINE/COLORADO/17864/2006(H3N8))	HEMAGGLUTININ, H3 HAEMAGGLUTININ HA1 CHAIN
2	B	HA2	polymer	175	20273.4	1	UniProt (E0UVR5) Pfam (PF00509) In PDB	INFLUENZA A VIRUS (A/CANINE/COLORADO/17864/2006(H3N8))	HEMAGGLUTININ, H3 HAEMAGGLUTININ HA2 CHAIN
3	C, D, F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
4	E	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7		water	water		18.0	3	Chemie (HOH)

Sequence modifications

A: 2 - 329 (UniProt: E0UVR5)

PDB	External Database	Details
Thr 30	Ser 45	engineered mutation

B: 1 - 172 (UniProt: E0UVR5)

PDB	External Database	Details
Ser 173	-	expression tag
Gly 174	-	expression tag
Arg 175	-	expression tag
Glu 131	Asp 475	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56633.4
Branched	Number of molecules	4
	Total formula weight	1859.7
Non-Polymers*	Number of molecules	3
	Total formula weight	413.3
All*	Total formula weight	58906.5

*Water molecules are not included.