4RTS

Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with AdoMet and a 5-bp non-canonical site (GTCTA)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA adenine methylase	polymer	298	34331.0	1	UniProt (H0Q7C9) Pfam (PF02086) UniProt (by SIFTS) (P0AEE8) In PDB	Escherichia coli
2	F	DNA (5'-D(*AP*CP*TP*TP*AP*GP*AP*CP*TP*TP*AP*G)-3')	polymer	12	3661.4	1		synthetic construct
3	G	DNA (5'-D(*TP*CP*TP*AP*AP*GP*TP*CP*TP*AP*AP*G)-3')	polymer	12	3661.4	1		synthetic construct
4	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
5		water	water		18.0	43	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: H0Q7C9)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 175	Ala 175	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	41653.8
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	42052.2

*Water molecules are not included.