4N2Z

Crystal Structure of the alpha-L-arabinofuranosidase PaAbf62A from Podospora anserina in complex with cellotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	GH62 arabinofuranosidase	polymer	360	40198.4	1	UniProt (E2GHW5) Pfam (PF03664) In PDB	Podospora anserina (Pleurage anserina)	alpha-L-arabinofuranosidase PaAbf62A
2	B	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	1	In PDB BIRD (PRD_900014) GlyTouCan (G03861VK)		alpha-cellotriose
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
6	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
7		water	water		18.0	370	Chemie (HOH)

Sequence modifications

A: 1 - 337 (UniProt: E2GHW5)

PDB	External Database	Details
Gly 338	-	expression tag
Leu 339	-	expression tag
Glu 340	-	expression tag
Gln 341	-	expression tag
Lys 342	-	expression tag
Leu 343	-	expression tag
Ile 344	-	expression tag
Ser 345	-	expression tag
Glu 346	-	expression tag
Glu 347	-	expression tag
Asp 348	-	expression tag
Leu 349	-	expression tag
Asn 350	-	expression tag
Ser 351	-	expression tag
Ala 352	-	expression tag
Val 353	-	expression tag
Asp 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40198.4
Branched	Number of molecules	1
	Total formula weight	504.4
Non-Polymers*	Number of molecules	4
	Total formula weight	638.8
All*	Total formula weight	41341.7

*Water molecules are not included.