4MOS

Pyranose 2-oxidase H450G/V546C double mutant with 2-fluorinated galactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pyranose 2-oxidase	polymer	633	70477.0	1	UniProt (Q7ZA32) Pfam (PF05199) In PDB	Trametes ochracea (White-rot fungus)	Pyranose oxidase
2	A	DIHYDROFLAVINE-ADENINE DINUCLEOTIDE	non-polymer		787.6	1	Chemie (FDA)
3	A	2-deoxy-2-fluoro-alpha-D-galactopyranose	non-polymer		182.1	1	Chemie (GAF)
4	A	2-deoxy-2-fluoro-beta-D-galactopyranose	non-polymer		182.1	1	Chemie (2FG)
5	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6		water	water		18.0	405	Chemie (HOH)

Sequence modifications

A: 1 - 622 (UniProt: Q7ZA32)

PDB	External Database	Details
Ala 2	Ser 2	SEE REMARK 999
Gly 450	His 450	engineered mutation
Cys 546	Val 546	engineered mutation
Ala 623	-	expression tag
Ala 624	-	expression tag
Ala 625	-	expression tag
Leu 626	-	expression tag
Glu 627	-	expression tag
His 628	-	expression tag
His 629	-	expression tag
His 630	-	expression tag
His 631	-	expression tag
His 632	-	expression tag
His 633	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	70477.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1347.1
All*	Total formula weight	71824.1

*Water molecules are not included.